Multispecific aspartate and aromatic amino acid aminotransferases in Escherichia coli

Characterization of Two Aromatic Amino Acid Aminotransferases and Production of Indoleacetic Acid in Azospirillum Strains

Sunnnary-Experiments were made to quantify indole-3-acetic acid (IAA) excreted by different wild type strains of Azospirilhun spp. The microorganisms produce IAA, during the late stationary growth phase and show a significant increase in IAA production when tryptophan is present in the medium. Under these conditions with the A. brusilense strain UAP 14, we observed two aromatic amino acid amino...

The amino acid pool in Escherichia coli.

A multispecific quintet of aromatic aminotransferases that overlap different biochemical pathways in Pseudomonas aeruginosa.

Pseudomonas aeruginosa possesses dual enzymatic sequences to both L-phenylalanine and L-tyrosine, a biosynthetic arrangement further complicated by the presence of five aromatic aminotransferases. Each aminotransferase is capable of transamination in vitro with any of the three keto acid intermediates in the aromatic pathway (phenylpyruvate, 4-hydroxyphenylpyruvate, or prephenate). The fraction...

Aspartate: 2-oxoglutarate aminotransferase from trichomonas vaginalis. Identity of aspartate aminotransferase and aromatic amino acid aminotransferase.

Aspartate: 2-oxoglutarate aminotransferase from the anaerobic protozoon Trichomonas vaginalis was purified to homogeneity and characterized. It is a dimeric protein of overall Mr approx. 100000. Only a single isoenzyme was found in T. vaginalis. The overall molecular and catalytic properties have features in common with both the vertebrate cytoplasmic and mitochondrial isoenzymes. The purified ...

Formation of aromatic amino acid pools in Escherichia coli K-12.

Phenylalanine, tyrosine, and tryptophan were taken up into cells of Escherichia coli K-12 by a general aromatic transport system. Apparent Michaelis constants for the three amino acids were 4.7 x 10(-7), 5.7 x 10(-7), and 4.0 x 10(-7)m, respectively. High concentrations (> 0.1 mm) of histidine, leucine, methionine, alanine, cysteine, and aspartic acid also had an affinity for this system. Mutan...